DOC Site Directed Mutagenesis Protocol - Brandeis University

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    Pfu Buffer 5µl ( pipet to mix well after this step. Pfu (2.5U/µl) 1µl ( mix well again PCR program (hot start): 95˚C 5 min. ... Site Directed Mutagenesis Protocol Author: Karen Daugherty Last modified by: Rosenstiel Center Created Date:

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