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Key words: nestin; dentate gyrus; input resistance; adult neurogenesis; neuronal precursor cells; ..... NeuN staining was observed in BrdU /nestin-GFP cells (Fig.
The Journal of Neuroscience, October 15, 2003 • 23(28):9357–9366 • 9357


Two Distinct Subpopulations of Nestin-Positive Cells in Adult Mouse Dentate Gyrus Satoshi Fukuda,1 Fusao Kato,2 Yusuke Tozuka,1 Masahiro Yamaguchi,3 Yusei Miyamoto,1 and Tatsuhiro Hisatsune1 1

Department of Integrated Biosciences, University of Tokyo, Kashiwa 277-8562, Japan, 2Laboratory of Neurophysiology, Department of Neuroscience, Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan, and 3Department of Physiology, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan

Neurogenesis in the dentate gyrus of the adult mammalian hippocampus has been proven in a series of studies, but the differentiation process toward newborn neurons is still unclear. In addition to the immunohistochemical study, electrophysiological membrane recordings of precursor cells could provide an alternative view to address this differentiation process. In this study, we performed green fluorescent protein (GFP)-guided selective recordings of nestin-positive progenitor cells in adult dentate gyrus by means of nestinpromoter GFP transgenic mice, because nestin is a typical marker for precursor cells in the adult dentate gyrus. The patch-clamp recordings clearly demonstrated the presence of two distinct subpopulations (type I and type II) of nestin-positive cells. Type I cells had a lower input resistance value of 77.1 M⍀ (geometric mean), and their radial processes were stained with anti-glial fibrillary acidic protein antibody. On the other hand, type II nestin-positive cells had a higher input resistance value of 2110 M⍀ and expressed voltagedependent sodium current. In most cases, type II cells were stained with anti-polysialylated neural cell adhesion molecule. Taken together with a bromodeoxyuridine pulse-chase analysis, our results may reflect a rapid and dynamic cell conversion of nestin-positive progenitor, from type I to type II, at an early stage of adult neurogenesis in the dentate gyrus. Key words: nestin; dentate gyrus; input resistance; adult neurogenesis; neuronal precursor cells; fluorescence microscopy

Introduction Accumulated evidence strongly supports the fact that neurogenesis occurs in the dentate gyrus (DG) of the adult mammalian hippocampus (Altman and Das, 1965, 1967; Kaplan and Hinds, 1977; Kaplan and Bell, 1984; Eriksson et al., 1998; Gould et al., 1998). New neurons in the adult DG become granule neurons and make functional synaptic connections with other neurons in vivo (van Praag et al., 2002). Until now, however, the origin of these new neurons has not been identified. In particular, there has been no report to date describing the functional characteristics, such as the electrophysiological properties, of early stage neuronal precursor cells in the adult DG. In the developing neocortex, neuroepithelial precursor–radial glia cells (Miyata et al., 2001; Noctor et al., 2001, 2002; Tamamaki et al., 2001) generate neurons at the ventricular zone. During the course of this neuronal differentiation, newborn neurons dra-

Received Jan. 21, 2003; revised Aug. 21, 2003; accepted Aug. 22, 2003. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (T.H.). T.H. is a recipient of grants from The Asahi Glass Foundation. Part of this study was supported by the Bioventure Project of the Ministry of Education, Culture, Sports, Science and Technology, Japan, to the Jikei University School of Medicine (F.K.). We thank Dr. Ron McKay for informative discussion and the gift of anti-nestin antibody, Dr. Tatsunori Seki for useful advice and the gift of anti-PSA-NCAM antibody, Dr. Christian Steinha¨user for critical reading of this manuscript, and Drs. Oliver Bru¨stle and Shigeo Okabe for informative comments on this study. Correspondence should be addressed to Dr. Tatsuhiro Hisatsune, Department of Integrated Biosciences, The University of Tokyo, Bioscience Building 402, 5-1-5 Kashiwanoha, Kashiwa Chiba 277-8562, Japan. E-mail: [email protected] Copyright © 2003 Society for Neuroscience 0270-6474/03/239357-10$15.00/0

matically elevate their input resistance (IR) to as much as ninefold higher than that of the precursor cells and begin to display the presence of voltage-dependent currents (LoTurco et al., 1991; Noctor et al., 2001). A similar scenario is suggested in DG by the presence of residual radial glia cells (Eckenhoff and Rakic, 1984). In this study, we have thus set out to directly assess membrane properties of neural precursor cells, especially at their early stage of differentiation. For this purpose, neural precursor cells in living brain slices must first be identified. We used nestin promoter– green fluorescent protein (GFP) transgenic mice (Yamaguchi et al., 2000) in which nestin second intron enhancers drive enhanced GFP. Nestin is a class IV intermediate filament protein (Hockfield and McKay, 1985; Frederiksen and McKay, 1988; Lendahl et al., 1990) and a marker for precursor cells in the adult DG (Palmer et al., 2000; Blu¨mcke et al., 2001; Nacher et al., 2001a). The nestin gene contains an enhancer in its second intron that directs expression specific to the CNS precursor cells (Zimmerman et al., 1994). We applied the technique of GFP-targeted selective recording (Auerbach et al., 2000) and directly analyzed the electrophysiological membrane properties of GFP-expressing precursor cells in living hippocampal slices. On the basis of analysis of the electrophysiological membrane properties in combination with morphological and immunohistochemical evaluation, we show here that the neural precursor cells expressing nestin in the DG can be classified into two distinct subpopulations. Preliminary results from this work have been presented previously in abstract form (Fukuda et al., 2002).

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Materials and Methods Animals. Nestin promoter–GFP transgenic mice were constructed by ligation of enhanced GFP cDNA (Clontech, Palo Alto, CA) with the nestin promoter region and the enhancer region of the nestin gene spanning the 3⬘ part of the second exon to the 5⬘ part of the third intron and the rabbit polyadenylation site (see Fig. 1 A) (Yamaguchi et al., 2000). Because we required a more intense GFP signal from the transgene to identify adult nestin-positive cells in acute hippocampal slices, we therefore used nestin promoter–GFP ⫹/⫹ (homozygote) mice. The age of mice used was from 6 weeks to 3 months [5-bromo-2-deoxyuridine (BrdU) pulse-chase experiments] or 6 months (other experiments). The nestin– GFP transgenic mice for BrdU pulse-chase experiments were injected with 15 mg/ml BrdU (Wako, Osaka, Japan) dissolved in 0.9% NaCl with 7 mM NaOH (final dose of 50 ␮g/gm body weight). All studies were performed in accordance with the Guidelines for Animal Experiments of the Graduate School of Frontier Sciences, University of Tokyo. Immunohistochemistry. The nestin–GFP mice were perfused with saline and 4% paraformaldehyde in PBS (in mM: 137 NaCl, 8.1 Na2HPO4, 2.68 KCl, 1.47 KH2PO4). Brains were postfixed at 4°C overnight in 4% paraformaldehyde in PBS and then kept at 4°C for a few days in 30% sucrose in Tris-buffered saline (TBS) (in mM: 137 NaCl, 2.68 KCl, 25 Tris). They were then embedded in OCT compound (Sakura, Tokyo, Japan) and cut by a cryostat (MICRON, Walldorf, Germany) into 40 ␮m sections. The slices were washed with TBS, pretreated with 0.5% Triton X-100 in TBS (Triton–TBS) for 30 min, and then blocked with blocking buffer (3% normal goat serum in 0.1% Triton–TBS) for 2 hr. Freefloating sections were incubated for 3 d at 4°C with rabbit polyclonal anti-nestin antibody (#130, rabbit-IgG, 1:200 dilution; a generous gift from Dr. Ron McKay, National Institute of Neurological Disorders and Stroke, Bethesda, MD) (Blu¨ mcke et al., 2001), mouse monoclonal antipolysialylated neural cell adhesion molecule (PSA-NCAM) antibody (12E3, mouse-IgM, 1:500 dilution; a generous gift from Dr. T. Seki, Juntendo University School of Medicine, Tokyo, Japan) (Seki and Arai, 1993; Seki 2002) and mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (mouse IgG; 1:200 dilution; Sigma, St. Louis, MO). After being washed in TBS, sections were incubated with rhodamineconjugated anti-rabbit IgG antibody (1:200 dilution; Chemicon, Temecula, CA), Cy5-conjugated goat anti-mouse IgM antibody (1:200 dilution; ICN, Costa Mesa, CA), and Cy5-conjugated anti-mouse IgG antibody (1:200 dilution; Jackson ImmunoResearch, West Grove, PA) and mounted with Immu-mount (Shandon, Pittsburgh, PA). Stained sections were analyzed with a confocal microscope (TCS SP2; Leica, Wetzlar, Germany) (Koketsu et al., 2003). For BrdU staining, the sections were incubated with 10 mM citric acid buffer at 90°C for 5 min. The heated sections were left at room temperature for 30 min and washed with TBS for 10 min. The sections were incubated in 1 M HCl at 37°C for 30 min, neutralized by rinsing with 0.1 M borate buffer, and washed with TBS. In addition to the above-mentioned first antibody, we used rat monoclonal anti-BrdU antibody (mouse IgG; 1:200 dilution; Harlan, Leicestershire, UK), rabbit polyclonal anti-GFP antibody (rabbit IgG; 1:1000 dilution; Molecular Probes, Eugene, OR), and mouse monoclonal anti-neuronal nuclei (NeuN) antibody (mouse IgG, 1:1000 dilution; Chemicon). For detection of the first antibody, rhodamine-conjugated anti-rat IgG antibody (1:200 dilution; Jackson ImmunoResearch), Alexa488-conjugated anti-rabbit IgG antibody (1:1000 dilution; Molecular Probes), and 7-amino-4-methylcoumarin-3-acetic acid (AMCA)conjugated anti-mouse IgG antibody (1:200 dilution; Chemicon) were used. Preparation of acute living slice. The nestin promoter–GFP transgenic mice used in the experiments were deeply anesthetized with ketamine Figure 1. GFP-expressing cells in the dentate gyrus of adult nestin promoter–GFP mice. A, Structure of nestin promoter–GFP transgene. The nestin promoter–GFP transgenic mouse expressed GFP under the regulatory element of nestin promoter and the second intron. B, The dentate gyrus of the nestin promoter–GFP mouse observed with the aid of a confocal microscope. C–F, The nestin immunostaining to the GFP-expressing cells with the process bearing GFAP. C, Merged image. D, GFP fluorescence; E, immunostaining for nestin; F, immunostaining for GFAP. The cell expressing nestin also expressed GFAP (arrows) and often comes in contact with blood vessels, which were also positively stained with anti-nestin (E; asterisk) as described

4 previously (Palmer et al., 2000), but this blood vessel did not express GFP signal. G–R, The three sets of the confocal image (G–J, K–N, and O–R) demonstrating the nestin immunostaining to the GFP-expressing cells bearing PSA-NCAM proteins at their cell surface (arrowheads). The merged image is shown in G, K, O; GFP fluorescence is shown in H, L, P; the immunostaining for nestin protein is shown in I, M, Q; the immunostaining for PSA-NCAM is shown in J, N, R. Note that the cell surface areas of PSA-NCAM-bearing GFP-positive cells were positively stained with this anti-nestin antibody. Scale bars: B, 100 ␮m; C–R, 10 ␮m.

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Figure 3. Visually guided patch-clamp recording from a cell expressing GFP in the adult DG of a nestin promoter–GFP mouse. A, Visual identification of a cell expressing GFP (arrow and arrowhead). B, Infrared DIC image of the slice. The cell in the center (arrow) was identified as GFP positive in A, and a patch pipette (seen from the left) was guided to the cell to establish the whole-cell configuration. C, Fluorescence image using the dye Alexa Fluor 568, which was spontaneously perfused from the patch pipette into the cell under recording. The wavelength of the excitation light used for the Alexa 568 was ⬃568 nm, and emission was in the red. Note that the emitted fluorescence signal is absent in another GFP-positive cell that was not patch clamped (arrowhead). D, Overlapped image of A and C certifying that the patch-clamp recording was made from a GFP-positive cell. Scale bar, 10 ␮m.

Figure 2. The BrdU pulse-chase examination on nestin–GFP transgenic mouse. A, The GFPpositive cell labeled with BrdU at the 2 hr time point. B–E, Separate image of A. B, Immunostaining for BrdU observed with rhodamine-conjugated anti-rat IgG antibody; C, immunostaining for GFP observed with Alexa488-conjugated anti-rabbit antibody; D, immunostaining for PSA-NCAM observed with Cy5-conjugated anti-mouse IgM antibody; E, immunostaining for GFAP observed with AMCA-conjugated anti-mouse IgG antibody. BrdU ⫹/GFP ⫹/GFAP ⫺/PSANCAM ⫹ cells are indicated with arrows. BrdU ⫹/GFP ⫹/GFAP ⫹/PSA-NCAM ⫺ cells are indicated with arrowheads. F, A BrdU-labeled cell stained with a neuronal marker, NeuN (arrow), didnotexpressGFPproteinatthe7dtimepoint.NeuNwasstainedwithCy5-conjugatedanti-mouse IgGantibody.J,PercentageofBrdU-labeledcellsinthesubgranularzoneatthetimepoint2,24,72hr, and7dafterBrdUinjection;144cellsfromthreeanimals(48pereachanimal)werecalculatedforeach group. In J, error bars indicate SE. Scale bars: A, 10 ␮m; F, 5 ␮m. (100 –150 mg/kg, i.p.) before decapitation. Brains were removed rapidly and placed in ice-cold low-calcium artificial CSF (ACSF) containing (in mM): 125 NaCl, 2.5 KCl, 0.1 CaCl2, 5 MgCl2, 1.25 NaH2PO4, 0.4 L-ascorbic acid, 25 NaHCO3 (osmolarity 320 mOsm/kg; pH 7.4 when saturated with 95% O2–5% CO2). For each experiment, the brain was glued to the cutting stage of a vibrating slice cutter (DTK-1000, Dosaka,

Kyoto, Japan) with the rostral side up and sliced into coronal sections (400 ␮m) that included the hippocampus. The slices were incubated in standard ACSF containing (in mM): 125 NaCl, 2.5 KCl, 2 CaCl2, 1.3 MgCl2, 1.25 NaH2PO4, 12.5 D-glucose, 0.4 L-ascorbic acid, 25 NaHCO3 (saturated with 95% O2–5% CO2) for 30 – 40 min at 37°C and then maintained at room temperature until recordings were performed (Miyakawa et al., 2002). One slice was fixed in a recording chamber (⬃0.2 ml volume, RC-26GLP; Warner Instruments, Hamden, CT) under nylon strings attached to a U-shaped platinum frame and then submerged in, and continuously perfused with, the standard ACSF at a flow rate of 1–2 ml/min (Kato and Shigetomi, 2001). The GFP-positive cells in the hippocampus were observed with the aid of a fluorescence microscope (BX50WI, Olympus, Tokyo, Japan) and visualized with a chilled chargecoupled device (CCD) video camera (C5985, Hamamatsu, Hamamatsu, Japan). After identification of the GFP signals, the light path of the microscope was switched to the infrared differential interference contrast (infrared DIC) optics mode. Infrared DIC images were monitored on a video monitor, which allowed visual guidance of the patch electrode (see below), and stored on the computer as image files together with a GFPfluorescence image (XL-20, Olympus) for morphological analyses and identification of the location of the recorded neuron. Whole-cell transmembrane current recordings. Patch electrodes were fabricated from borosilicate glass capillaries of 1.2 mm outer diameter (1B120F-4, World Precision Instruments, Sarasota, FL) with a programmable puller (P-87, Sutter Instrument, Novato, CA). The composition of the pipette internal solution was (in mM): 120 potassium gluconate, 6 NaCl, 5 CaCl2, 2 MgCl2, 2 MgATP, 0.3 NaGTP, 10 EGTA, 10 HEPES, pH 7.2 with KOH. The intracellular solution also included Lucifer yellow dilithium salt [0.5%; Sigma) or Alexa Fluor 568 hydrazide sodium salt (0.5%; Molecular Probes)] for visual identification of the cells recorded. The tip resistance of the electrodes ranged from 5 to 10 M⍀ when filled with this solution. After establishment of the cell-attached configuration (1–10 G⍀ seal resistance), the whole-cell mode was established with negative pressure and negative current pulses using a DigiData 1200

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membrane potentials were appropriately corrected. Cells with an RMP above ⫺30 mV were rejected. Data were recorded at room temperature with an Axopatch 200B patch-clamp amplifier (Axon Instruments) or with a CEZ-2400 amplifier (Nihon-kohden, Tokyo, Japan). The membrane current was sampled on-line at 4 kHz (PowerLab, AD Instruments, Grand Junction, CO) after filtering at 2 kHz and stored on the hard disk of a personal computer. The data were analyzed off-line with “Igor Pro 4.01” (WaveMetrics, Lake Oswego, OR). External test solution was made up in standard ACSF and often was included with 1 mM 4-aminopyridine (4-AP) (Sigma) or 1 ␮M tetrodotoxin (TTX) (Wako). Immunostaining for recorded slices. After recording, some slices were postfixed at 4°C overnight in 4% paraformaldehyde in TBS and then kept at 4°C for a few days in 30% sucrose in TBS. The slices were washed with TBS, pretreated with 0.5% Triton X-100 in TBS for 30 min, and then blocked with blocking buffer. Free-floating sections were incubated for 3 d at 4°C with PSA-NCAM antibody or GFAP antibody as mentioned above.


Figure 4. Bimodal distribution of input resistance in nestin–GFP-positive cells. A, Relationship between IR and RMP. Abscissa, IR in logarithmic scale; ordinate, RMP. Plot of results from a total of 74 GFP-positive cells. B, Distribution of IR of GFP-positive cells. Ordinate, number of cells. Abscissa, IR (same as A). The curve indicates the result of a nonlinear, least squares curve fitting of a double Gaussian distribution. The vertical broken line indicates the trough between two Gaussian peaks at ⬃500 M⍀. C, Distribution of IR for GFP-negative mature neurons in the DG. interface (Axon Instruments, Foster City, CA) controlled by a pCLAMP6 (Axon Instruments) program. The IR and resting membrane potential (RMP) were measured immediately after rupture of the patch membrane to avoid possible changes in the membrane properties attributable to cytoplasmic perfusion with the pipette solution. The IR was calculated by measuring the steady-state passive current evoked with a hyperpolarizing pulse of 5 mV amplitude and 10 msec duration repeated every 40 msec under voltage-clamp mode from a holding potential of ⫺70 mV (see Fig. 5 A, B). The IR value measured by this 10 msec pulse was comparable with the IR value estimated by the longer (600 msec) pulse measurement ( p ⬍ 0.001; n ⫽ 20), in which the value of IR was estimated using the two current values at the end of ⫺40 and ⫺50 mV voltage holds (see Fig. 5C,D). The averaged values of series resistance on “type I” and “type II” as measured by the pCLAMP6 membrane test mode were 13.9 ⫾ 1.4 M⍀ (n ⫽ 10; mean ⫾ SE) and 14.4 ⫾ 0.3 (n ⫽ 10), respectively. The series resistance and membrane capacitance were not compensated in all the recording. RMPs were measured by switching the amplifier to the current-clamp mode with no current injection. The liquid junction potentials were ⬃10 mV as calculated with Clampex 8.2 (Axon Instruments), and then all

GFP-positive cells in adult DG from nestin–GFP mice can be classified into two subpopulations In a previous study, we described that nestin promoter–GFP transgenic mice exhibited a large number of GFP-positive cells within the subgranular zone of the adult hippocampal DG (Fig. 1), and these GFP-positive cells were stained with GFAP or PSANCAM (Yamaguchi et al., 2000). In this communication, we first confirmed that both types of nestin–GFP-positive cells would express nestin protein. For this purpose, we used polyclonal antinestin antibody (#130), which was kindly provided by Dr. Ron McKay (Blu¨ mcke et al., 2001). As shown in Figure 1C–F, the process of the GFP-positive cell bearing GFAP-positive process was stained strongly by this antinestin antibody. Similar staining has been observed in most of the GFP-positive cells bearing GFAP (Fig. 1G–J, arrows). On the other hand, the nestin immunoreactivity to the GFP-positive cell bearing the PSA-NCAM molecules was relatively weak and limited to the surface of its cell membrane. We showed several examples of this type of staining (Fig. 1G–R). As shown in Figure 1G–J, the cell surface of GFP-positive cells bearing PSA-NCAM including their proximal process was stained significantly by this anti-nestin antibody. The level of GFP signal was relatively weaker than that of surrounding PSA-NCAM-negative GFPpositive cells, presumably GFP-positive cells bearing GFAP, suggesting decreased nestin-promoter activity in GFP-positive cells bearing PSA-NCAM. Figure 1 K–R demonstrates three other examples of the cell surface expression of nestin proteins in GFPpositive cells bearing PSA-NCAM. We performed such a nestin staining study very extensively and could conclude that GFPpositive cells bearing PSA-NCAM express nestin protein to a significant level. The set of nestin staining data strongly suggests that GFP expression in nestin–GFP transgenic mice would correspond to the expression of nestin protein in adult DG. Because nestin-positive cells can be distinguished on the basis of GFAP or PSA-NCAM expression, we evaluated whether one or both cell types are active in the process of neurogenesis. Figure 2 A–E shows a four-color immunostaining for distinguishing each cell type (GFAP ⫹ vs PSA-NCAM ⫹) in the BrdU pulse-chase experiments (Fig. 2 J). At 2 hr after BrdU injection, the frequency of GFP ⫹/GFAP ⫹/PSA-NCAM ⫺ in total BrdU ⫹ cells was 27.7%, whereas 54.2% of BrdU ⫹ cells was GFP ⫹/GFAP ⫺/PSANCAM ⫹. In this analysis, we also observed BrdU ⫹/GFP ⫹/ GFAP ⫺/PSA-NCAM ⫺ cells, and the frequency was 13.2%. At 24 hr after BrdU injection, the percentage of GFP ⫹/GFAP ⫹/PSANCAM ⫺ cells decreased to 15.9%, while that of GFP ⫹/GFAP ⫺/

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ure 3 illustrates how selective recording from GFP-positive cells was made. GFPpositive cells were identified (Fig. 3A) by observing their fluorescence signals displayed on a video monitor. The optical path was then changed to infrared DIC (Fig. 3B), which permitted the tip of the patch electrode to be positioned on the cell body of a GFP-positive cell (Fig. 3B, arrow). After establishment of the whole-cell configuration, the intracellular solution in the patch pipette spontaneously and gradually perfused into the cytoplasm of the cell, which was confirmed by observing the fluorescence signal of the hydrophilic fluorescent dye in the patch pipette solution emanating from the tip of the patch electrode and filling the cell (Alexa Fluor 568 in the case of Fig. 3C). In this way, we confirmed the patch recording of the GFPpositive cells from the observation that the cell shape visualized with injected fluorescence dye exactly overlapped with the shape from GFP signal (Fig. 3D). Immediately after the establishment of the whole-cell configuration, the IR and RMP were measured (Fig. 4 A). The IR of GFP-positive cells was extremely variable, ranging from 22.6 to 8460 M⍀. Interestingly, the distribution of the IR for these cells was clearly bimodal (Fig. 4 B). When the number of cells was plotted as a function of the logarithmic value of IR (Fig. 4 B), the resulting frequency histogram could be fit with a double Gaussian distribution with two distinct peaks (Fig. 4 B, solid line). The estimated value of the two Gaussian peaks was 10 (1.86 ⫾ 0.42)(␮ ⫾ ␴) Figure 5. Electroresponsiveness of type I (lower IR) and type II (higher IR) GFP-positive cells in the DG. A, C, E, Results from type M⍀ for the left peak and 10 (3.34 ⫾ 0.36) M⍀ I cells; B, D, F, results from type II cells. A, B, Measurement of IR with a ⫺5 mV square voltage pulse (above) from a holding for the right peak. A statistic indicates that potential around ⫺70 mV. The steady-state current at the end of the hyperpolarizing command pulse was measured. Current these Gaussian peaks are independent traces (below) are averages of the responses to 100 consecutive pulses. C, D, Responses to a voltage step pulse indicated at the top ( p ⬍ 0.05; Student’s t test), thus showing of the panel. Four current traces are superimposed for each panel. Note that the vertical scales are different between C and D. E, F, Plot of the transmembrane current (ordinates) elicited by the voltage step pulses shown in C and D against the command voltage that the nestin-positive cells can be classi(abscissas). Open circles represent the values at the peak of the outward current (C, D); filled circles indicate the steady-state fied into two distinct subpopulations on the basis of their IR. These Gaussian peaks current values (C, D). were also significantly ( p ⬍ 0.05; Student’s t test) different from the Gaussian PSA-NCAM ⫹ cells increased to 78.5%. At 72 hr after BrdU inpeak fit to the IR distribution for mature GFP-negative neurons jection, the percentage of GFP ⫹/GFAP ⫹/PSA-NCAM ⫺ cells recorded from the same slices (Fig. 4C) (10 (2.31 ⫾ 0.29) M⍀; n ⫽ 30 still decreased to 9.7%. These results strongly suggested cell difcells). In effect, the two peaks of the IR distribution split at ⬃500 ferentiation from GFAP ⫹/PSA-NCAM ⫺ nestin-positive “early M⍀. The RMPs (Fig. 4 A, ordinate) of the cells belonging to the progenitors” to GFAP ⫺/PSA-NCAM ⫹ nestin-positive “late protwo distinct peaks in the IR distribution were significantly differgenitors” in adult dentate gyrus. Neuronal differentiation of ent [⫺80.4 ⫾ 2.3 and ⫺69.7 ⫾ 2.9 mV (mean ⫾ SE) for the cells nestin-positive progenitors was subsequently analyzed with antiwith smaller IR and larger IR, respectively; p ⬍ 0.05; Student’s t NeuN staining of BrdU ⫹ cells. BrdU ⫹/NeuN ⫹ cells first aptest] (Fig. 4 A). On the basis of these results, we termed the cells peared at 72 hr after BrdU injection. At 7 d after BrdU injection, with smaller IR (⬍500 M⍀) as type I cells and those with larger IR NeuN staining was observed in BrdU ⫹/nestin-GFP ⫺ cells (Fig. (⬎500 M⍀) as type II cells. The geometric mean for the recorded 2 F–I ), and its proportion was 20%. Taken together, these results IR data of type I cells was 77.1 M⍀ (refer to 10 (1.89 ⫾ 0.27)(mean ⫾ SD) ⫹ ⫹ ⫺ may indicate that nestin /GFAP /PSA-NCAM early progeniM⍀ from n ⫽ 44). This value is significantly smaller than that of tors would differentiate into nestin ⫹/GFAP ⫺/PSA-NCAM ⫹ late type II cells (2110 M⍀; refer to 10 (3.32 ⫾ 0.27) M⍀ from n ⫽ 30) progenitors and then produce newborn neurons at adult DG. ( p ⬍ 0.001; Student’s t test), again indicating that these subpopuPatch-clamp recordings of whole-cell membrane currents lations of GFP-positive cells belong to distinct groups of cells. The from visually identified GFP-positive cells were performed. Figratio of cell numbers between type I and type II cells shown above

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may not correctly reflect the actual proportion of these cell types in the adult DG, for the ease of giga-seal formation depends on the cell type. In this recording study, we were able to exclude data from vascular endothelial cells, which occasionally express nestin–GFP signal (data not shown), from the two criteria. The vascular endothelial cells can be easily identified from their typical cell shape. Moreover, if recorded intentionally, the RMP of these cells was above ⫺20 mV (data not shown), a value excluded from the present analyses (see Materials and Methods). Voltage-dependent membrane currents of type I and type II cells Figure 5 illustrates the results of further analyses of the electrophysiological characteristics of the GFP-positive cells. First, type I and type II cells were identified by measuring the passive current elicited by identical voltage steps (Fig. 5 A, B). The steady-state current was used to estimate IR as plotted in Figure 4. Figure 5C indicates that in the type I cell tested, a slowly inactivating outward current, reminiscent of the A-current, is expressed in response to depolarizing pulses (open circle) delivered after a prepulse to ⫺100 mV from ⫺70 mV for 400 msec. This outward cur- Figure 6. Comparison of membrane currents among type I GFP-positive cells, type II GFP-positive cells, and GFP-negative rent was activated at membrane potentials mature neurons. A, D, Recordings and results from a type I cell. B, E, Recordings and results from a type II cell. C, F, Recordings and above ⫺60 mV (Fig. 5E, open circles) and results from a mature GFP-negative granule neuron. A–C, Spontaneous recording of the membrane current recorded at a holding inactivated to reach a steady-state level at current of ⫺70 mV. Note that mature granule neuron exhibits spontaneous EPSCs, whereas in the type II cell such spontaneous 800 msec (Fig. 5C,E, closed circles). This activity is absent. D–F, Responses to depolarizing voltage step pulses. The command voltage protocol is shown at the top of D and was the same for E and F. Pipette capacitance and series resistance were not compensated. No type I cells exhibited voltageoutward conductance was observed in 25 activated inward current ( D). Note the expression of rapidly rising and rapidly inactivating inward currents (arrowhead) during of 31 type I cells. The component of depolarization in E, which is much smaller and slower than that in F (arrowhead). The voltage-dependent inward currents on type voltage-activated outward current at ⫺40 II GFP-positive cells were completely blocked by the addition of 1 ␮M TTX (n ⫽ 10 of 10) ( E). The values of IR and series resistance mV was mostly [78.9 ⫾ 2.2% (mean ⫾ were monitored before and after these recordings (Fig. 6 E). The last values of IR and series resistance were within 90 –110% of the SE); n ⫽ 4] inhibited by 1 mM 4-AP, sug- initial values. gesting the presence of A-current in type I cells. In contrast, rapidly inactivating outblocked by the addition of TTX (Fig. 6 E). In the GFP-negative ward currents (Fig. 5D, open circle) was more pronounced in mature neurons, the fast inward current was much larger (Fig. type II cells. This outward current was activated significantly 6 F, arrowhead). These observations clearly demonstrate that above ⫺50 mV and inactivated within 50 msec (Fig. 5 D, F ). The type II cells express functional voltage-dependent fast sodium same voltage step protocol elicited a very similar, rapidly inacticonductance. vating outward current in four of five type II cells tested. Contrary to our initial expectation when we started to record Type I and type II nestin-positive cells in the adult DG have from GFP-positive cells in these transgenic mice, type I and type different morphological and immunohistochemical II cells presented neither spontaneous postsynaptic currents nor characteristics action potentials during depolarization (Fig. 6 A, B) [for type I To evaluate the morphological features of GFP-positive cells in (n ⫽ 44) and type II (n ⫽ 30) cells, respectively]. This was not the DG in relation to the above classification, we measured the caused by the recording conditions because all GFP-negative malength of the principal process of nestin–GFP-positive cells. The ture granule neurons recorded in the same slices (n ⫽ 30) preprocess length of each cell is plotted against IR in Figure 7A. sented spontaneous postsynaptic currents (Fig. 6C) and action Interestingly, most type I cells had processes ⬎10 ␮m, whereas potentials. As shown in Figure 6 D, no type I cells exhibited GFP-positive processes were rarely detected for type II cells (Fig. voltage-activated inward current (n ⫽ 14); however, type II cells 7 B, C). The length of the process in type I cells was significantly showed voltage-activated inward current (Fig. 6 E, arrowhead). longer than that of type II cells ( p ⬍ 0.05; Mann–Whitney U This current was activated mostly at membrane potential ⫺30 test), indicating that these subgroups have distinct morphologimV. In 6 of 24 type II cells, we obtained “net” inward current cal characteristics. exceed the holding current at ⫺80 mV, and the average of these The fluorescence of Lucifer yellow, which filled the cell during six currents from the holding potential of ⫺80 mV was 32.5 ⫾ the whole-cell recording, was visualized using confocal micros10.0 pA (mean ⫾ SE). These inward currents were completely copy. Type I cells had a triangular soma and a thick process ex-

Fukuda et al. • Nestin-Positive Cells in Adult Mouse Dentate Gyrus

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Figure 7. Relationship between process length and IR in GFP-positive cells. A, The length of the principal process with GFP signal was measured in all GFP-positive cells recorded using a fluorescence microscope with a high-sensitivity chilled CCD camera. Abscissa, IR in logarithmic scale; ordinate, process length. The maximum length was limited to 50 ␮m because of the measurement on the TV monitor of the fluorescence-sensitive CCD camera. Filled diamonds indicate data from type I cells; open diamonds indicate type II cells. B, C, Representative examples of the fluorescence image of a type I cell (B) and a type II cell (C). Note that the type I cell exhibits a process,whereasthetypeIIcelldoesnot.Also,notethattheGFPfluorescencesignalisweakerintype II cells, which was a commonly observed feature of these cells. Scale bar, 10 ␮m.

tending radially to the molecular layer, with several branching processes (Fig. 8 A, B). These morphological properties are reminiscent of those reported for “residual radial glial cells” (Kosaka et al., 1986; Nacher et al., 2001a). In contrast, type II cells had a round soma and a very thin process (Fig. 8C,D) that were not visible in the GFP images. The direction of the type II cell process was opposite or tangential to the molecular layer of the DG. These morphological features of type I and type II cells were clearly different from those of mature astrocytes (Fig. 8 E) and mature granule neurons (Fig. 8 F) in the DG. Morphological properties highly similar to those described above were observed in nine type I and five type II cells in which a successful postfixation analysis was made after the patch-clamp recording. In a limited number of patched cells, it was possible to immunohistochemically analyze the expression of specific markers in the cells identified to be type I or type II. Presumably because of a lack of cell– cell contact, the patched soma could usually be lifted from the slice during removal of the patch pipette in most of the cells tested, especially in the type II cells. As shown in Figure 9, all type I cells (n ⫽ 9/9) were stained with GFAP, and most of type II cells (n ⫽ 6/8) were stained with PSA-NCAM. These data indicate that type I and type II cells classified by the electrophysiological method correspond to the groups of GFAP-positive and PSANCAM-positive cells (Figs. 1, 2), respectively. The remaining type II cells (n ⫽ 2 of 8) were not stained with PSA-NCAM, and we have shown GFP ⫹/GFAP ⫺/PSA-NCAM ⫺ cells in Figure 2J. Therefore, GFP ⫹/GFAP ⫺/PSA-NCAM ⫺ cells are probably classified into type II cells. These postfixation studies indicated that type I nestin-positive cells express GFAP proteins and type II nestin-positive cells start to express PSA-NCAM molecules.

Figure 8. Morphological features of type I and type II nestin-expressing cells examined using fluorescence imaging after injection of Lucifer yellow into the cell during the whole-cell patchclamp recording. The slices were fixed after the recording, and the fluorescence was detected and visualized with a confocal scanning microscope. A, Type I cell; B, another type I cell; C, a type II cell; D, another type II cell; E, a GFP-negative typical astrocyte in dentate gyrus; F, a GFPnegative typical granule neuron in dentate gyrus. Note that type I cells (A, a small type I cell; B, a large type I cell) always extended their principal process toward the molecular layer as same as granule neurons of dentate gyrus ( F), whereas the typical astrocytes extended their processes in all directions (Fig. 8 E). Type II cells extended their processes to the opposite side of ( C) or tangentially to ( D) the granule cell layer. Scale bars, 15 ␮m.

Discussion This study is the first demonstration of the physiological properties of nestin-positive precursor cells in living hippocampal slices from mature animals. Using hippocampal slices from mature transgenic nestin promoter–GFP mice (Yamaguchi et al., 2000), we have analyzed the electrophysiological properties and morphological characteristics of the nestin-expressing DG cells by selectively recording whole-cell membrane currents from GFPpositive cells. Recently, Kempermann et al. (2003) also used this nestin promoter–GFP mouse for the study on adult neurogenesis. In our study, we have first confirmed the validity of the visualization of immature precursor cells (Figs. 1, 2). The new findings of the present study can be summarized as follows (Fig. 10). (1) The nestin-positive cells can be classified clearly into two distinct subpopulations according to their difference in various phenotypes: type I and type II nestin-positive cells. (2) Type I cells presented a smaller IR (from 22.6 to 350 M⍀). On the other hand, type II cells presented a larger IR (from

9364 • J. Neurosci., October 15, 2003 • 23(28):9357–9366

Figure 9. Expression of specific markers in type I and type II nestin-positive cells as confirmed by post-recording immunohistochemistry. Confocal microscopy images from slices in which a patch-clamp recording from a type I (IR ⫽ 36.0 M⍀) ( A–D) and a type II (IR ⫽ 910 M⍀) ( E–H) cell was made. A, Merged image of the images shown in B–D; B, GFP fluorescence; C, fluorescence image of Alexa Fluor 568 injected into the cell during whole-cell recording; D, immunostaining for GFAP observed with Cy5-conjugated anti-mouse IgG antibody, which was used to avoid cross-talk with other fluorescent emissions but shown in blue to demonstrate costaining with other dyes. E, Merged image of the images presented in F–H; F, GFP fluorescence (note the fluorescence intensity of this type II cell is weaker than the surrounding GFPpositive cells); G, fluorescence image of Alexa Fluor 568 injected into the cell during whole-cell recording; H, immunostaining for PSA-NCAM. Scale bars: A–D, 10 ␮m; E–H, 5 ␮m.

Figure 10. A schematic diagram illustrating the two types of nestin-positive progenitors at an early stage of neurogenesis in adult dentate gyrus. Cell morphology was on the basis of the images of the recorded cells injected with fluorescence dye (Figs. 8, 9). The BrdU pulse-chase analysis (Fig. 2) strongly suggested that GFAP ⫹ type I and PSA-NCAM ⫹ type II would be early and late progenitors for newborn neurons at adult DG. SGZ, Subgranular zone; GCL, granular cell layer; ML, molecular layer.

532 to 8460 M⍀) and mostly exhibited depolarization-activated inward sodium current. (3) Type I cells were characterized by their longer processes toward the molecular layer, being stained with anti-GFAP, whereas most type II cells showed the expression of PSA-NCAM at their surface. (4) The BrdU pulse-chase analysis (Fig. 2) strongly suggested that type I and type II nestin-positive cells would be regarded as early progenitor and late progenitor, respectively. The key issue now, as discussed below, is the cellular identity of type I and type II cells and their role in adult neurogenesis.

Fukuda et al. • Nestin-Positive Cells in Adult Mouse Dentate Gyrus

The variation in the population of nestin-positive cells in the DG reflects variations in distinct differentiation stages during neurogenesis. In this context, we favor the view that type I cells as early progenitors are “radial glia-like” precursors that generate type II cells as late progenitors possessing some neuronal properties. The observation that the GFP signal of type II cells was generally weaker than other surrounding type I cells (Fig. 9) could support this scenario. It is reasonable to presume that early progenitor type I cells and late progenitor type II cells could be comparable with “B cells” and “D cells,” which were named in the previous study (Seri et al., 2001). Seri et al. (2001) have also demonstrated that the proportion of B cells of BrdU-positive cells decreases dramatically at 24 hr after BrdU injection, whereas that of D cells increases, indicating a rapid transition from B cells to D cells. Our BrdU pulse-chase experiments have also strongly supported this theory: a rapid and dynamic shift from GFAP-positive early progenitors to PSA-NCAM-positive late progenitors. By means of extensive double immunostaining with antiPSA-NCAM and anti-GFAP of DG of adult nestin promoter transgenic mice, we have observed few (⬍1%) nestin-positive cells, which were stained with PSA-NCAM at a proximal site and seemed to possess GFAP ⫹ fiber at a distal site (data not shown). On the other hand, we detected many GFAP ⫺/PSA-NCAM ⫺/ BrdU ⫹ nestin-positive cells. This type of cell is presumably classified into type II cell and is possibly in a transient state between GFAP ⫹ and PSA-NCAM ⫹ nestin-positive cells. We subsequently discuss the phenotypes of type I nestinpositive cells and type II nestin-positive cells (see below). The type I cells were characterized by their relatively low IR (mean, 77.1 M⍀; range, 22.6 –350 M⍀). This range substantially overlaps that for recordings from DG astrocytes in hippocampal slices of adult rats (mean, 175 M⍀; range, 119 –278 M⍀) (Bordey and Sontheimer, 2000), although, in the developmental stage, the IR of glial cells is relatively high (Kressin et al., 1995; Matthias et al., 2003). The relatively more negative RMP of type I cells in the present study suggests that the low IR is attributed to a larger resting potassium conductance as in the case of astrocytes (McKhann et al., 1997; Bordey and Sontheimer, 2000). In developing neocortex, radial glia cells have large conductance attributed their gap junction (Noctor et al., 2001), but this would be not the case in our study because the gap junction-permeable dyes Alexa and neurobiotin did not spread to other neighboring cells and the gap junction inhibitor halothane did not increase the value of IR in type I cells (data not shown). The expression of a depolarizationactivated, slowly inactivating outward current exclusively in type I cells is not inconsistent with the report by Bordey and Sontheimer (2000) who detected A-current-like outward currents in 82% of the DG astrocytes that they examined. In addition, the cells confirmed to be type I by patch-clamp recordings also costained with GFAP, thereby strongly supporting the notion that type I cells represent a subgroup of astroglia in the DG. The shape of the type I cells, however, was not exactly the same as that of typical astrocytes. Rather, the morphological features of type I cells, such as radial GFAP-positive projections toward the molecular layer and a triangular soma, are quite similar to those of residual radial glia cells demonstrated in the adult DG (Kosaka and Hama, 1986; Alvarez-Buylla et al., 1987; Schmidt-Kastner and Szymas, 1990; Cameron et al., 1993; Seki and Arai, 1999). These radial glia cells with GFAP indeed express nestin (Fig. 1) (Nacher et al., 2001a). These types of radial glia are shown to be involved in the formation of the DG in rats and monkeys (Eckenhoff and Rakic, 1984; Rickmann et al., 1987) in a manner similar to the situation of neurogenesis in the developing neocortex.

Fukuda et al. • Nestin-Positive Cells in Adult Mouse Dentate Gyrus

This suggests that type I cells might play a neuronal precursor role similar to that played by radial glia cells in cortical neurogenesis (Noctor et al., 2001). Seri et al. (2001) have shown, using the cell-specific integration of a retroviral vector, that GFAP-positive radial glia cells generate new neurons in the DG of adult rats and have proposed that radial glia cells act as neuronal precursor cells. The type I cells of our study share many morphological properties with the B cell (Seri et al., 2001), further suggesting that type I cells are early progenitors in the DG. A surprising result in the present study was the extremely high IR of the type II cells, which ranged from 532 M⍀ to 8.46 G⍀. The IR of mature granule neurons, measured by whole-cell recordings, has been reported to be 388 M⍀ in adult mouse (van Praag et al., 2002), 182–248 M⍀ in adult rat (Wang et al., 2000), 261.8 M⍀ in young rat (Mott et al., 1997), and 202 M⍀ (this study). Therefore the IR of type II cells measured here does not fall into any documented range. Such a high value of IR in DG has not been reported in any study except that by Liu et al. (2000), in which the relationship between morphological maturity and electroresponsive properties of DG granule cells was analyzed. They reported in developing rat DG that the IR of morphologically immature granule cells is extremely high (2–3 G⍀), whereas that of mature neurons is significantly lower (100 –350 M⍀). Noctor et al. (2001) reported that in developing neocortex, the IR of migrating neurons outside of the DG was as high as 1.03 G⍀. Our study also demonstrated that type II cells expressed voltage-gated inward currents and were costained with PSANCAM, a specific marker for immature neurons in the hippocampus (Seki and Arai, 1993; Kuhn et al., 1996; Nacher et al., 2001b). Taking our results together with those of several other studies (Palmer et al., 2000; Blu¨ mcke et al., 2001; Gu et al., 2002) in which expression of nestin in neuronal precursor cells with a morphology similar to the type II cells in this study, we propose that type II cells are late progenitors at an early stage of neuronal differentiation in the adult DG. The expression of the neuron-like fast inward current in type II cells is strong evidence supporting the possibility that type II cells serve as neuronal progenitor cells. In conclusion, the use of the nestin promoter–GFP transgenic mouse has permitted us to characterize two distinct subpopulations of nestin-positive cells in the DG of mature animals, which might have important implications in developing our understanding of the process of neurogenesis in the adult brain.


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